Ortega RM1, Jiménez A1, Andrés P2, Faci M1, Lolo JM1, Lozano MC1, Bermejo LM1, López-Sobaler AM1, Requejo AM1

1 Departamento de Nutrición. Facultad de Farmacia, Universidad Complutense de Madrid. Spain.
2 Departamento deTécnicas Instrumentales. Facultad de Farmacia, Universidad Complutense de Madrid. Spain.

Correspondence address: Profa. Dra. Rosa M. Ortega.
Departamento de Nutrición, Facultad de Farmacia, Universidad Complutense, 28040-Madrid (Spain). Telephone: 34-1-3941837. Fax: 34-1-3941732. E-mail: rortega@eucmax.sim.ucm.es

Background: Serum homocysteine levels are a risk factor in cardiovascular disease. Knowledge on how dietary factors might affect these levels is therefore of interest.
Objective: To evaluate serum homocysteine levels in a group of elderly people and analyse the effect of pyridoxine, vitamin B12 and folic acid intakes on these levels.
Design: The study subjects were 130 independently-living elderly people over the age of 65. A dietetic study was performed using a 7-day food record. Serum homocysteine levels were determined by HPLC.
Results: Mean pyridoxine, vitamin B12 and folate intakes were 67.2±16.8%, 392.8±549.2% and 84.5±28.3% of recommended values respectively. With regard to sex, differences were seen only for vitamin B12 levels (9.1±12.7 g/day in men, and 6.5±8.8 g/day in women). Some 93.6% of subjects showed pyridoxine intakes below those recommended, as did 17.6% with respect to vitamin B12 and 72.8% with respect to folic acid. Homocysteine levels were 12.4 mol/l (12.6±3.7 mol/l in men and 12.2±7.9 mol/l in women) (P<0.05). No significant differences were seen in homocysteine levels between subjects with lower than recommended intakes of pyridoxine or vitamin B12 and those with better intakes. However, subjects with folic acid intakes below 200 g/day showed higher homocysteine levels (13.0±6.7 mol/l) than did subjects with more adequate intakes (10.9±4.1 mol/l) (P<0.05).
Conclusion: The diet of the study subjects might be improved, especially with respect to pyridoxine and folic acid. Raising the intake of the latter might be especially useful in controlling homocysteine levels and the risk of cardiovascular disease.

Keywords: Serum homocysteine, elderly, folate intake, pyridoxine intake, cyanocobalamin intake, observational study